Agglutination grade

Sperm Agglutination Classification. The grading system is as follows: Grade 0: no agglutination; Grade 1, Isolated: <10 sperm cells per agglutinate; Grade 2, Moderate: 10-50 sperm cells per agglutinate; Grade 3, Strong: > 50 sperm cells; Grade 4, Complete: completely agglutinated spermatozoa; What causes agglutination Agglutination is the clumping of particles. The word agglutination comes from the Latin agglutinare. Agglutination is the process that occurs if an antigen is mixed with its corresponding antibody called isoagglutinin. This term is commonly used in blood grouping. This occurs in biology in two main examples: The clumping of cells such as bacteria or red blood cells in the presence of an antibody or complement. The antibody or other molecule binds multiple particles and joins them, creating a la

Coagglutination Test When the specific antibodies (agglutinins) bind to surface antigens of bacteria/virus or any antigens immobilized in particulate matter (such as latex particle) and cause the formation of a visible clumps, such test is called agglutination test. Agglutination test can be performed in Agglutination is often used by your body's immune system to clump toxins or pathogens together. Once congregated, these invaders can easily be inactivated by cells in the immune system To value the heap there are grades that range from 0 (no agglutination) up to 3 (which means that there are more than 30%-40% of adhered spermatozoa). Causes of agglutination The agglutination suggests the presence of antibodies in ejaculation

What is Sperm Agglutination? - Definition from FertilitySmart

Agglutination of Bacteria and Viruses. The use of agglutination tests to identify streptococcal bacteria was developed in the 1920s by Rebecca Lancefield working with her colleagues A.R. Dochez and Oswald Avery. [1] She used antibodies to identify M protein, a virulence factor on streptococci that is necessary for the bacteria's ability to cause strep throat Agglutination 1. + AGGLUTINATION Dr Rania Abo-Shady Ain Shams University 2. Tests Based on Ag/Ab Reactions • All tests based on Ag/Ab reactions will have to depend on lattice formation or they will have to utilize ways to detect small immune complexes • All tests based on Ag/Ab reactions can be used to detect either Ag or A • The result of the tests are scored from 0 to 4+, ie, 0 (no agglutination), 1+ (25% agglutination), 2+ (50% agglutination), 3+ (75% agglutination) or 4+ (100% agglutination). The smallest quantity of serum that exhibits a 2+ or 50% agglutination is considered the end-point of serum activity or titre. O > 1 in 80 H > 1in 160 14 Agglutination reaction is an immunological assay, which results in the specific antigen and antibody reaction in vitro. Agglutination purely refers to the clumping or aggregation of the particles. As a result of agglutination, a cross-linked structure or lattice appears in the form of visible aggregates.. Agglutination reaction is a sensitive, easy and cost-effective method to carry out and. #Grading_agglutination #DMLT #Star_tutorial how to grade agglutination reaction how to create blood group after blood group test 1 + 2 + 3 + 4 + and zero grading is.

Agglutination. Agglutination is defined as the formation of clumps of cells or inert particles by specific antibodies to surface antigenic components (direct agglutination) or to antigenic components adsorbed or chemically coupled to red cells or inert particles (passive hemagglutination and passive agglutination, respectively) 69 sentence examples: 1. Conclusion: injection can improve high agglutination condition. 2. It is also known as the gelatin agglutination test. 3. Use data of high polymer colophony, agglutination at base material underside, have stability and moist prepared test tube is ____ to grade the agglutination. Serologic centrifuge Good lighting Magnification mirror. Process utilization of ___ is very important in GRADING of agglutination. 3 WASSERMAN TEST TUBE. Test tube used and quantity of tube. 0.85-0.9% isotonic saline. What is used for washing Agglutination refers to the gluing or clumping of particles or cells in the presence of an agglutination antibody. Agglutination techniques are useful in detecting antigen-antibody reactions. The agglutination reaction reflects many types of interactions that can take place between certain molecules when they get together and form a complex Agglutination And RH factors Agglutination. Sometimes when the blood of 2 people is mixed together, it clump or forms visible island in the liquid plasma the red cell becomes attached to one another. this agglutination when different types blood are mixed within the body the reaction can be bursting of the red cells as well as agglutination different types of blood are recognized on the.

Agglutination is a linguistic process pertaining to derivational morphology in which complex words are formed by stringing together morphemes without changing them in spelling or phonetics. Languages that use agglutination widely are called agglutinative languages. An example of such a language is Turkish, where, for example, the word evlerinizden, or from your houses, consists of the morphemes ev-ler-iniz-den, literally translated morpheme-by-morpheme as house-plural-your-from. Agglutinativ шкала агглютинаци

1+ agglutination: Red blood cell button breaks into many small clumps barely visible macroscopically; background is turbid; many free red blood cells. Negative: No agglutinated red blood cells present; red cells are observed flowing off the red blood cell button during the process of grading Latex agglutination test basically a rection between an antigen and an antibody. When they react, a visible clumping appears. This clumping is called agglutination. This helps to diagnosing the including Hepatitis B, Meningitis and so on. Its protocol is really quick and it shows its results in 15 minutes to an hour Agglutination reactions where the antigens are found naturally on a particle are known as direct agglutination. In active agglutination, direct agglutination of particulate antigen with specific antibody occurs. Direct bacterial agglutination uses whole pathogens as a source of antigen

Agglutination, a grammatical process in which words are composed of a sequence of morphemes (meaningful word elements), each of which represents not more than a single grammatical category.This term is traditionally employed in the typological classification of languages. Turkish, Finnish, and Japanese are among the languages that form words by agglutination Other articles where Agglutination is discussed: blood group: The importance of antigens and antibodies: antibodies usually results in clumping—agglutination—of the red cells; therefore, antigens on the surfaces of these red cells are often referred to as agglutinogens Agglutination definition is - the action or process of agglutinating. 3: the formation of derivational or inflectional words by putting together constituents of which each expresses a single definite meanin The intensity of the agglutination indicates the concentration of antibody in the serum. Sometimes strong agglutination reactions need to be confirmed by heating the sera (56 C. for 30 minutes) to destroy non-specific agglutinins or by repeating the test with various dilutions of the serum. 60.5.2 Slow agglutination in tubes/tube agglutination A group of passive agglutination tests carried out by coating either antigen or antibody on an artificial carrier particle, called latex bead are called as latex agglutination test. They may alternatively referred to as latex fixation test and may be:. Latex Agglutination Test (LAT) for Antibody Detection: A Passive Agglutination Test with Antigen bound to the surface of latex beads.

The method is highly sensitive, and the degree of agglutination is directly proportional to the concentration of free antibody against RBC antigenic determinants and inversely proportional to the amount of antigen. A modification of this technique, for the purpose of detecting an infection, is the indirect or passive hemagglutination assay Zetadex-50 Agglutination Grade is a beaded composite density gradient gel developed by emp Biotech. A highly purified gel grade, Z-50AG exhibits narrow size distribution and high transparency, allowing flexible use with Gel Cards for a wide variety of tests (AHG, ABO-Rh, Kell) Agglutination is the process whereby specific antigens (e.g., red blood cells) aggregate to form larger visible clumps when the corresponding specific antibody is present in the serum. Figure 10-1 Agglutination patterns. A, Slide agglutination of bacteria with known antisera or known bacteria Parasite density is estimated based on World Health Organization recommended average amastigote density grading of slides , grade 1 to 6. The Direct agglutination test (DAT, Institute of Tropical Medicine—Antwerp (ITM), Belgium) was conducted with a freeze-dried version of the DAT antigen composed of fixed, trypsin-treated and stained. PH. * numerical value assigned to degree/strength of agglutination. In the United Kingdom, grade 5 is used for maximum strength instead of 4. In other laboratories a plus sign may be used instead of a numeral, the number of plus signs indicating the degree of strength of the reaction, e.g. +, ++, or +++

Agglutination (biology) - Wikipedi

  1. The Second Stage (Agglutination). (1) Once RBCs are sensitized, they may or may not directly agglutinate. Blood group antibodies were characterized empirically before the immunoglobulin. classes were recognized. Those antibodies that could produce agglutination in a saline
  2. Sperm agglutination modarate grade 2 on sperm test results What does this mean in terms of conceiving naturally? - Answered by a verified OB GYN Doctor. We use cookies to give you the best possible experience on our website
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background. about us. laborator red blood cell button and examine for agglutination. 7.17 Grade and record the results on the antibody identification panel. 7.18 Wash tubes a minimum of 3 times with isotonic saline. Completely decant saline after final wash to obtain a dry red cell button. 7.19 Add two drops of AHG or Anti-IgG to each tube If you are positive for the Rh protein, you just denote a positive sign (+) after the identified ABO type (e.g. B+ indicates that agglutination occurred in both the anti-B and the anti-D sera. Unfortunately, there are a number of variations in the D protein and it is possible to get false negatives (i.e. you are listed as a Rh negative person, but in fact are Rh positive)

A semen analysis (plural: semen analyses), also called seminogram, or spermiogram evaluates certain characteristics of a male's semen and the sperm contained therein. It is done to help evaluate male fertility, whether for those seeking pregnancy or verifying the success of vasectomy.Depending on the measurement method, just a few characteristics may be evaluated (such as with a home kit) or. La seizième leçon de cette méthode d'apprentissage s'intéresse à l'agglutination des clés glidées et à la suppression de la lettre W.Le grade correspondant à la maîtrise de cette leçon est le grade 2.5. À l'issue de cette leçon vous devriez être à même d'agglutiner tous les types de clés et de vous faire économiser un peu de place

Video: Agglutination Tests: Types, principle and uses - Learn

Find 2021 quality & cheap agglutination for sale. You can get inexpensive agglutination with discounted price from the most reputable wholesalers The agglutination test was performed by mixing the fusion protein with red blood cells and the serum of a patient recovered from COVID-19 (specimen collected at least 28 days post symptoms and negative PCR testing upon discharge). Successful manufacturing of clinical grade SARS-CoV-2 specific T cells for adoptive T cell therapy When affected people's blood is exposed to cold temperatures (32º to 50º F), certain proteins that normally attack bacteria (IgM antibodies) attach themselves to red blood cells and bind them together into clumps (agglutination)

Agglutination in Hematology: Definition & Examples Study

Incidental vaginal low-grade squamous intraepithelial neoplasia was noted. Later, the lesion progressed and was confirmed to be condyloma acuminata. No recurrence of labial agglutination was noted 3 months after the surgery. We emphasize that surgical intervention should be the first consideration for labial agglutination with urinary symptoms. Brucella total antibody titer of greater than or equal to 1:160 by standard tube agglutination test (SAT) or Brucella microagglutination test (BMAT) in one or more serum specimens obtained after onset of symptoms Detection of Brucella DNA in a clinical specimen by PCR assay NOTE: Evidence of Brucella antibodies by nonagglutination-based test A latex agglutination kit (LA)* and an indirect hemag- glutination kit (1HA)t are commercially available and vague, low-grade clinical signs of disease for weeks and may have reached their ceiling IgG titer when initially tested making the documentation of an increasing tite A Coombs test, also known as antiglobulin test (AGT) is either of two blood tests used in immunohematology.They are the direct and indirect Coombs tests. The direct Coombs test detects antibodies that are stuck to the surface of the red blood cells. Since these antibodies sometimes destroy red blood cells, a person can be anemic and this test can help clarify the condition Record the results of agglutination with grading. 8. Wash the cells 3 to 4 times with saline, decant completely after each wash and add 2 drops of antihuman globulin serum to the sediment cells. Shake the tubes to mix the contents and then centrifuge at 1500 rpm for 1 minute. Examine for agglutination and grade the agglutination reaction

What Is Agglutination in Sperm? - Causes, Symptoms & Treatmen

BIOL 640 1st Edition Lecture 12 Outline of Last Lecture I Double immunodiffusion assay Outline of Current Lecture II Immunoelectrophoresis III Hemag (a) Direct agglutination test: Diluted serum of patients is tested by slide or tube method against a brucella suspension. A titre of 80 IU or more is an indication of infection, latent active or past infection (Table): Prozone phenomenon (absence of agglutination in low dilution of serum due to high concentration of antibodies is common and hence a serial dilutes of serum is to be tested Lymphocyte Agglutination - 2. #00003287. Category: Lymphoma: Mature B-cell and Plasma cell Neoplasms > Low-grade B-cell lymphoma > Follicular Lymphoma Published Date: 07/01/2007 Flow cytometric analysis of the total white cell population demonstrated CD5 − /CD10 +.

Agglutination. Agglutination is a 13 letter word, used as a noun, and has the letters aaggiilnnottu (agilnotu). Starts with a, ends with n, seven consonants, six vowels and five syllables INTRODUCTION — Cold agglutinin disease (CAD) is a form of autoimmune hemolytic anemia (AIHA) in which cold agglutinins (agglutinating autoantibodies with an optimum temperature of 3 to 4°C) can cause clinical symptoms related to agglutination of red blood cells in cooler parts of the body and hemolytic anemia.. This topic will review the pathophysiology, clinical manifestations, diagnosis. The majority of these false positives show a low grade of agglutination, though up to 1 percent of these results may demonstrate higher grades of agglutination. In another study consisting of a cohort of healthy, non-hospitalized individuals, the incidence of a positive DAT result without evidence of hemolysis was found to be 0.1 percent. Buy and get information for Latex Agglutination Teaching Kit, HTI001, Molecular Biology, HiPer® Teaching Kits, Immunolog

Agglutination Assays Microbiolog

  1. X Hu ABO cells) + control sera, agglutination grade Human IgG1, IgG2, IgG3, IgG4, Sheep anti-, Set of 4 Ab's, precipitation grade Goat Serum (normal), without NaN3 (sodium azide) Mouse.
  2. Polyagglutination 27 describes agglutination of red cells by all or most normal adult sera but not by the patient's own serum. This is the result of IgM antibodies reacting with an antigen on the red cells, which is usually hidden but can be exposed by enzyme activity
  3. The clumping together of suspended material resulting from the action of AGGLUTININS. | Explore the latest full-text research PDFs, articles, conference papers, preprints and more on AGGLUTINATION.
  4. Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) | Explore the latest full-text research PDFs, articles.
  5. Some languages have longer words than others -- but that's not just a simple choice. There's a lot of different ways to mix up morphemes, even if they all me..

After the screening test of the undiluted serum samples, if the agglutination grade was 1+ or higher, the reaction mixtures were titrated (1:2 through 1:1,024). Statistical analysis. Receiver operating characteristic (ROC) curve analysis was performed to determine the cutoff for positivity that would give the optimal sensitivity and specificity The importance of antigens and antibodies. The red cells of an individual contain antigens on their surfaces that correspond to their blood group and antibodies in the serum that identify and combine with the antigen sites on the surfaces of red cells of another type. The reaction between red cells and corresponding antibodies usually results in clumping— agglutination—of the red cells.

Agglutination - SlideShar

Sperm agglutination occurs when the body's immune system recognizes its own sperm as foreign and deploys sperm antibodies to fight it. The antibodies attach themselves to the sperm forcing them to clump together. When the sperm are coated by the antibodies, their mobility becomes reduced and their receptors, which allow the sperm and egg to. agglutination. The research method was experimental by performing blood type with drops of serum in the blood type A, B and AB then saw the grade agglutination. The data obtained were processed statistically using Kruskal Wallis. The result showed serum A, B, and O can be use Human IgG2, precipitation grade Human IgG3 (min X Hu ABO cells), agglutination grade Human IgG3, precipitation grade Human IgG4 (min X Hu ABO cells), agglutination grade Human IgG4, precipitation grade Rabbit Immunoglobulin, FITC Control Serum, Human, as reference in immuno-electrophoresis Human IgG1, IgG2, IgG3, IgG4, Sheep anti-, Set of 4 Ab. Use for latex and other agglutination tests; Customized layout is possible Bottles are made of medical grade HDPE.

5. Examine the agglutination reaction in each tube by dislodging the button gently. If necessary, use a magnifying hand lens. 6. Interpretation: Agglutination will be recognised by the formation of small clumps in a clear liquid. As the bottom of the test tube is tapped, the clumps whirl up and then settle down Activation of helper T cel

Medical Dictionary is intended for use by healthcare consumers, students, and professionals as well as anyone who wants to keep up with the burgeoning array of terminology found in today's medical news. By staying clear of jargon, the dictionary offers fast and concise information, whether the user is searching for a description of an over-the-counter or prescription medication, a medical. Growth rate in culture media and lectin mediated agglutination tests were done to study three Leishmania major Strains. These strains were the Senegallese strain MHOM / SN / 00 / DKI (SN), the Sudanese strain MHOM I SD / UG8 (SD) and the Saudi strain NHOM / SA / 84/ JtSH (SA). The in vitro behavior of the three L major strains was traced in. Apr 6, 2015 - This pattern is similar to that seen in the previous slide, except that the reactivity at the antiglobulin phase is much weaker (w+ indicates weakly positive, or between agglutination grade 0 and 1). The autocontrol is again negative. Such a pattern is suggestive of a high titer, low avidity antibody. These antibodies are directed against high frequency antigens

What is Agglutination Reaction? Definition, History, Steps

Widal reaction. When is considered a negative Widal exam? (a) 1/40 (b) 1/80 (c) 1/160 (d) 1/320 e) none of the options. Widal reaction in Qualitative method (on plate) as quantitative method (in tube) the method of launching is the same Agglutination Agglutination AlphaLISA AlphaLISA Array Tomography Array Tomography Biacore Biacore Binding Binding experiments Biosensors Biosensors Block Blocking experiments BN-PAGE Blue native polyacrylamide gel electrophoresis CE Capillary Electrophoresis cELISA In cell ELISA Cell Culture Cell Culture CGH Comparative genomic hybridization ChI Reporting of antiglobulin agglutination test results can be on a qualitative or quantitative basis. For qualitative methods, the interpreter examines the test tube and assigns a score based on a graded scale: M: Mixed field - any degree of agglutination in a sea of non-agglutinated cells. W: tiny aggregates, turbid reddish background Immunology & Serology in Laboratory Medicine (5th Edition) Edit edition. Problem 22M24 from Chapter 10: Match each grade of agglutination with its respective descri... Get solution Bacterial agglutination test Example of agglutination test. Measure the antibody produced by the host against bacterial agglutinins. Best performance when used in sterile physiologic saline. Uses: Disease diagnosis: bacterial agent is difficult to cultivate in vitro Example of agglutination test. Some examples of such diseases are: Tetanus: Clostridium tetani

Grading agglutination blood bank ll ABO Blood grouping ll

agglutination - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. see thi The doctor(GP) said they found non specific agglutination > 10 %, however he did not give me any answers: Moderate Liquefaction: Incomplete Non Specific Agglutination >10 % Motility 65% Progressive 58% Immotile 35.

All of the answers given so far are not quite correct, but give great data, so I am going to use it. In the simplest manner possible: in an agglutinative language, each word bit that you put on the root in order to have the word fit the sentence c..

Agglutination - an overview ScienceDirect Topic

PPT - CLLS 3311 Advanced Clinical Immunohematology

Clumps of red cells suspended in a clear fluid. Agglutination in tube test is graded from 1+ to 4+ and read macroscopically (See Figure). Figure 785.1: Grading of ABO tube test CAD in 90% of patients is an immunoglobulin M (IgM) -mediated process, with rare findings of monoclonal IgG, IgA, or λ light chain restriction, whereas warm AIHA is predominantly an IgG-driven disease. 5,14,15 The term cold agglutinin arises less from the clinical symptomatology and instead refers to the finding of agglutination without antiglobulin antisera in microtiter wells at 4°C Agglutination that occurs when AHG (antihuman globulin) is added indicates that the specific antibodies are attached (in-vivo) on the red cell antigen. Direct Coombs Test is used to demonstrate in-vivo coating (in-vivo sensitization) of red cells with antibodies or complement in particular, IgG and C3d reacted with IgG antibodies only, and the tube agglutination test was equally effective in detecting antibodies of both classes of im-munoglobulins. INTRODUCTION The serologic tests used most commonly for detecting anti-bodies against Mycoplasma meleagridis in turkeys are the serum plate agglutination and tube agglutination tests (3,6,8,13).

Agglutination in a sentence (esp


Grade 3 or 4 neutropenia was seen in one-third of patients, but only 11% developed an infection. Rituximab/bendamustine is highly efficient and safe and could be considered as first-line therapy for cold agglutinin disease. 25,2 for agglutination. 7.25 At least a grade 2 reaction is expected following the addition of IgG sensitized cells. If agglutination following the addition of IgG sensitized cells is weaker than expected or not detected the test is invalid and must be repeated. 7.26 Grade and record results. 7.27 Interpret and report antibody screen results Several types exist: a) Hemagglutination of RBC's b) Bacterial Agglutination c) Passive Agglutination d) Agglutination Inhibition Immunologic tests: 4) Radioimmunoassay (RIA)- very sensitive test; used for measuring hormones, serum proteins, drugs, etc. at low [C]'s (≤ .001ug/ml) measures competitive binding of radiolabelled Ag. Neisseria meningitidis is a leading cause of bacterial meningitis and septicemia in children and young adults in the United States. Rapid and reliable identification of N. meningitidis serogroups is crucial for judicious and expedient response to cases of meningococcal disease, including decisions about vaccination campaigns. From 1997 to 2002, 1,298 N. meningitidis isolates, collected in the. Agglutination Agent Raw Materials Acrylic Acid Cas No:79-10-7 , Find Complete Details about Agglutination Agent Raw Materials Acrylic Acid Cas No:79-10-7,Acrylic Acid Cas No 79-10-7,Agglutination Agent Raw Materials,79-10-7 from Syntheses Material Intermediates Supplier or Manufacturer-Labeyond Chemicals (Dalian) Co., Ltd

Agglutination Antibodies - Antibodies by Application

Grade 8 students identified their blood groups today in the lab, applying what they learned in class about agglutination reaction that occurs between specific antigens and antibodies. Share. Leave a comment Cancel Reply. Save my name, email, and website in this browser for the next time I comment Antibody definition is - any of a large number of proteins of high molecular weight that are produced normally by specialized B cells after stimulation by an antigen and act specifically against the antigen in an immune response, that are produced abnormally by some cancer cells, and that typically consist of four subunits including two heavy chains and two light chains —called also. Nov 21, 2019 - Acute Myeloid Leukemia (AML) is a malignant proliferation of myeloblasts in the blood and bone marrow

Agglutination And RH factors Notes, Videos, QA and Tests

agglutination grade — шкала агглютинации Возможные однокоренные слова agglutinating — слипающийся, склеивание, агглютинация, агглютинирующи Home ← SHMS - Saudi OER Network Home. Refine your search. Education Standard ليستيريا لاتكس مجموعة اختبارات طبّية , Find Complete Details about ليستيريا لاتكس مجموعة اختبارات طبّية,مجموعة اختبارات طبّية لاتكس from Other Lab Supplies Supplier or Manufacturer-Guangdong Huankai Microbial Sci. & Tech. Co., Ltd The panel suggests targeted genetic testing over low-dose ristocetin-induced platelet agglutination (RIPA) to diagnose type 2B VWD for patients suspected of type 2A or 2B in need of additional testing panel developed and graded the recommendations and assessed the certainty in the supporting evidence following the GRADE approach. 4-9,29,30.

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Agglutination - Wikipedi

Study free Laboratory Science flashcards and improve your grades. Matching game, word search puzzle, and hangman also available Grade of agglutination (positive) 1/20 /, 1/40,1/80,1/160,1/320,1/640. No type of agglutination degree-Negative. I mean if you don't have any degree of agglutination, we should launch negative if you present, you'll throw the numbers above (depending on the degree of agglutination) Formulated with a premium-grade ingredient Tyrostat, appearance of skin pigmentations and age spots will be limited and stopped, therefore treating Melasma! These results indicate that glutathione inhibits the synthesis and agglutination of melanin by interrupting the function of L-DOPA.It is the master anti-oxidant that helps fight free. Grade B: 10% %Grade C: 05 Grade D: 30% total abnormal forms: 25% Agglutination: Present R.B.C : 1-2 spermatogenic cells : 12-15 round cells: 25-30 pus cells: 15-20 هل التحليل كويس وهل نسبة الصديد عالية واذا كانت عالية ما علاجها ؟؟. Grade A ≥25% or Grade A+B ≥ 50% within 60 minutes Grade A Sperm Motility % Grade B Grade C Grade D Total Progressive motile (sperm count / ejaculate) ≥ 20 million / ejaculate Normal Sperm Morphology % ≥ 50% Round cells (WBCs + Germ Cells ) <5 cells /HPF Sperm Agglutination % < 10% Others : RBCs , Epithelial Cells Nil/HP

Test Tube Agglutination ViewerNS20-2113 ABO-Rh Blood Typing Using Neo/BLOOD
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